Catalog Number - 65308-05P

Zx Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octyl (C8) bonded phase. Designed as a cost-effective alternative to Agilent Zorbax® C8 columns, it offers comparable chromatographic behavior for USP L7 methods while providing the moderate hydrophobic retention, reproducibility, and reliability expected from a conventional C8 stationary phase. Compared to C18 columns, C8 phases generally provide reduced retention and faster elution of hydrophobic analytes.

65308-05P is a 50 mm x 4.6 mm ID analytical column packed with 3 µm Zx Similars C8 particles. The short 50 mm length is optimized for rapid analyses, fast equilibration, and high-throughput workflows. The 3 µm particle size provides significantly higher efficiency and improved peak capacity compared to traditional 5 µm columns, while generating higher operating pressures that should be considered during method development. Typical operating flow rates are approximately 1.0–2.0 mL/min on HPLC systems capable of supporting the increased backpressure associated with smaller particle packings.

This USP L7 column is ideal for hydrophobic and neutral compounds when less retention is desired than a comparable C18 phase. Common applications include pharmaceutical analysis, long-chain fatty acid separations, quality control testing, and fast analytical methods requiring chromatographic behavior similar to Agilent Zorbax® C8 columns.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Agilent Zorbax® C8 columns

• Fast analysis with 50 mm column length

• Higher efficiency than comparable 5 µm columns

• Lower retention than C18 columns

• Excellent for hydrophobic and neutral compounds

• End-capped C8 bonded phase

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Method development

• Long-chain fatty acid analysis

• Fast analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Zx Similars C8 Octyl

• USP Classification: L7

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Short column length provides rapid analyses

• Higher efficiency than comparable 5 µm columns

• Increased operating pressure compared to larger particle sizes

• Compatible with HPLC systems capable of handling elevated backpressure

• Provides less retention than comparable C18 stationary phases

• Excellent alternative for many USP L7 methods

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